📋1. OVERVIEW & PROTOCOL

• Compound Structure: TB-500 is supplied as the acetate salt of Thymosin Beta-4, a 43-residue, water-soluble synthetic peptide belonging to the beta-thymosin family.
• Biological Focus: Evaluated in laboratory systems to analyze actin-sequestration biochemistry, characterization of monomeric G-actin pools, and cell-migration and scratch-wound assays.
• Cellular Intensity: Preclinical investigations probe endothelial and fibroblast migration, closure dynamics in cultured monolayers, and angiogenesis-associated tissue-remodeling signaling pathways.

🧪2. CHEMICAL PROPERTIES

• Chemical Formula: C212H350N56O78S
• Molar Mass: 4693 g/mol
• CAS Number: 77591-33-4
• Analytical Standard: Identity confirmed by mass spectrometry (ESI-MS or MALDI-TOF) verifying sequence fidelity against theoretical values, identifying single methionine residue oxidation states.
• Shelf Life & Stability: 36 months as a lyophilized freeze-dried white powder; hygroscopic and sensitive to repeated temperature cycling.
• Available Variations: 10mg 1 Vial | 10mg 3 Vials | 10mg 5 Vials | 10mg 10 Vials

🔬3. AUDITED RESEARCH & SPECIFICATIONS

• Stability Advantage: Supplied strictly as a characterized reference substance for controlled in-vitro and rodent-model laboratory work, structured to withstand process-related impurities.
• Signaling Pathways: Mediates interactions through a short conserved segment commonly written as the LKKTET-type motif to evaluate cytoskeletal dynamics at the bench.
• TB-500 Comparison: Contrasts full-length peptide structures with shorter actin-binding fragments in comparative fragment studies to map specific residue drive and molecular outcomes.